So far, many investigators are exploring HSCs and modified HSCs for use in clinical and basic science. Among many stem cells, the feasibility of using adult-derived hematopoietic stem cells in therapeutic approaches is very diverse, and is unarguably regarded as an important cell source in stem cell biology. Mantel CR, O'Leary HA, Chitteti BR, Huang X, Cooper S, Hangoc G, Brustovetsky N, Srour EF, Lee MR, Messina-Graham S, Haas DM, Falah N, Kapur R, Pelus LM, Bardeesy N, Fitamant J, Ivan M, Kim KS, Broxmeyer HE (2015) Enhancing hematopoietic stem cell transplantation efficacy by mitigating oxygen shock.Hematopoietic stem cells (HSCs) are regarded as one of essential cell sources for treating regenerative diseases. Oguro H, Ding L, Morrison SJ (2013) SLAM family markers resolve functionally distinct subpopulations of hematopoietic stem cells and multipotent progenitors. J Exp Med 211:1315–1331īoggs DR (1984) The total marrow mass of the mouse: a simplified method of measurement. Gazit R, Mandal PK, Ebina W, Ben-Zvi A, Nombela-Arrieta C, Silberstein LE, Rossi DJ (2014) Fgd5 identifies hematopoietic stem cells in the murine bone marrow. Simonnet AJ, Nehme J, Vaigot P, Barroca V, Leboulch P, Tronik-Le Roux D (2009) Phenotypic and functional changes induced in hematopoietic stem/progenitor cells after gamma-ray radiation exposure. Mayle A, Luo M, Jeong M, Goodell MA (2013) Flow cytometry analysis of murine hematopoietic stem cells. Cell 121:1109–1121Įrgen AV, Jeong M, Lin KK, Challen GA, Goodell MA (2013) Isolation and characterization of mouse side population cells. Kiel MJ, Yilmaz OH, Iwashita T, Yilmaz OH, Terhorst C, Morrison SJ (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Blood 105:2717–2723įorsberg EC, Prohaska SS, Katzman S, Heffner GC, Stuart JM, Weissman IL (2005) Differential expression of novel potential regulators in hematopoietic stem cells. Yang L, Bryder D, Adolfsson J, Nygren J, Mansson R, Sigvardsson M, Jacobsen SE (2005) Identification of Lin(-)Sca1(+)kit(+)CD34(+)Flt3- short-term hematopoietic stem cells capable of rapidly reconstituting and rescuing myeloablated transplant recipients. Immunity 15:659–669Ĭhristensen JL, Weissman IL (2001) Flk-2 is a marker in hematopoietic stem cell differentiation: a simple method to isolate long-term stem cells. Science 273:242–245Īdolfsson J, Borge OJ, Bryder D, Theilgaard-Monch K, Astrand-Grundstrom I, Sitnicka E, Sasaki Y, Jacobsen SE (2001) Upregulation of Flt3 expression within the bone marrow Lin(-)Sca1(+)c-kit(+) stem cell compartment is accompanied by loss of self-renewal capacity. Osawa M, Hanada K, Hamada H, Nakauchi H (1996) Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Osawa M, Nakamura K, Nishi N, Takahasi N, Tokuomoto Y, Inoue H, Nakauchi H (1996) In vivo self-renewal of c-Kit+ Sca-1+ Lin(low/-) hemopoietic stem cells. Okada S, Nakauchi H, Nagayoshi K et al (1992) In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells. ![]() ![]() Ikuta K, Weissman IL (1992) Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Spangrude GJ, Heimfeld S, Weissman IL (1988) Purification and characterization of mouse hematopoietic stem cells. This chapter outlines techniques for HSC identification and analysis from mouse bone marrow postirradiation. These include hematopoietic stem cells (HSCs), which are central to blood reconstitution and life-long bone marrow function, and are important targets of analysis in these studies. Among these complications is a radiation-induced loss of c-Kit, a central marker for conventional gating of primitive hematopoietic populations in mice. While flow cytometry methods are well characterized for identification and analysis of bone marrow populations in the nonirradiated setting, multiple complications arise when dealing with irradiated tissues. The study of these processes, as well as the development of treatments to prevent hematopoietic damage or enhance recovery after radiation exposure, often require analysis of bone marrow cells early after irradiation. Radiation exposure is particularly damaging to cells of the hematopoietic system, inducing pancytopenia and bone marrow failure.
0 Comments
Leave a Reply. |